Details

<p>Preface xi</p> <p>About the Companion Website xiii</p> <p><b>Chapter 1 Proteins and proteomics 1</b></p> <p>1.1 Proteins, an introduction 1</p> <p>1.2 Genes, genomics and proteomics 2</p> <p>1.3 Bioinformatics 12</p> <p>1.4 Proteomics: goals and applications 14</p> <p>Further reading 22</p> <p><b>Chapter 2 Protein structure and engineering 25</b></p> <p>2.1 Primary structure 25</p> <p>2.2 Higher-level structure 36</p> <p>2.3 Protein classification on the basis of structure 41</p> <p>2.4 Protein structural stability 45</p> <p>2.5 Higher-order structure prediction 47</p> <p>2.6 Protein folding 48</p> <p>2.7 Intrinsically disordered proteins 50</p> <p>2.8 Protein engineering 51</p> <p>2.9 Protein post-translational modification 54</p> <p>Further reading 62</p> <p><b>Chapter 3 Protein sources 65</b></p> <p>3.1 Recombinant versus non-recombinant production 65</p> <p>3.2 Approaches to recombinant protein production 67</p> <p>3.3 Heterologous protein production in E. coli 72</p> <p>3.4 Heterologous production in bacteria other than E. coli 77</p> <p>3.5 Heterologous protein production in yeast 77</p> <p>3.6 Heterologous protein production in fungi 78</p> <p>3.7 Proteins from plants 80</p> <p>3.8 Animal tissue as a protein source 84</p> <p>3.9 Heterologous protein production in transgenic animals 85</p> <p>3.10 Heterologous protein production using animal cell culture 86</p> <p>3.11 Insect cell culture systems 87</p> <p>Further reading 88</p> <p><b>Chapter 4 Protein purification and characterization 91</b></p> <p>4.1 Protein detection and quantification 93</p> <p>4.2 Initial recovery of protein 95</p> <p>4.3 Removal of whole cells and cell debris 98</p> <p>4.4 Concentration 103</p> <p>4.5 Chromatographic purification 107</p> <p>4.6 Protein inactivation and stabilization 128</p> <p>4.7 Protein characterization 137</p> <p>Further reading 139</p> <p><b>Chapter 5 Large-scale protein production 141</b></p> <p>5.1 Upstream processing 141</p> <p>5.2 Downstream processing 154</p> <p>5.3 Therapeutic protein production: some special issues 163</p> <p>5.4 Range and medical significance of impurities potentially present in protein-based therapeutic products 166</p> <p>Further reading 175</p> <p><b>Chapter 6 Therapeutic proteins: blood products, vaccines and enzymes 177</b></p> <p>6.1 Blood products 177</p> <p>6.2 Anticoagulants 184</p> <p>6.3 Thrombolytic agents 186</p> <p>6.4 Additional blood-related products 189</p> <p>6.5 Vaccine technology 190</p> <p>6.6 Therapeutic enzymes 194</p> <p>Further reading 202</p> <p><b>Chapter 7 Therapeutic antibodies 205</b></p> <p>7.1 Antibodies 205</p> <p>7.2 IgG structure and activity 205</p> <p>7.3 Antibody therapeutics: polyclonal antibody preparations 209</p> <p>7.4 Antibody therapeutics: monoclonal antibodies 211</p> <p>7.5 Therapeutic applications of monoclonal antibodies 220</p> <p>7.6 Antibody conjugates 223</p> <p>7.7 Bispecific antibodies 224</p> <p>7.8 Antibody fragments 225</p> <p>7.9 Engineering the antibody glycocomponent 228</p> <p>7.10 Fc fusion proteins 229</p> <p>Further reading 230</p> <p><b>Chapter 8 Hormones and growth factors used therapeutically 233</b></p> <p>8.1 Insulin 233</p> <p>8.2 Glucagon 240</p> <p>8.3 Gonadotrophins 240</p> <p>8.4 Growth hormone 243</p> <p>8.5 Erythropoietin 246</p> <p>8.6 Other hormones 247</p> <p>8.7 Growth factors 249</p> <p>Further reading 253</p> <p><b>Chapter 9 Interferons, interleukins and tumour necrosis factors 257</b></p> <p>9.1 Regulatory factors: cytokines versus hormones 257</p> <p>9.2 Interferons 258</p> <p>9.3 Interleukins 264</p> <p>9.4 Tumour necrosis factors 271</p> <p>Further reading 274</p> <p><b>Chapter 10 Proteins used for analytical purposes 277</b></p> <p>10.1 The IVD sector 279</p> <p>10.2 The basis of analyte detection and quantification 280</p> <p>10.3 Enzymes as diagnostic/analytical reagents 281</p> <p>10.4 Biosensors 289</p> <p>10.5 Antibodies as analytical reagents 295</p> <p>Further reading 309</p> <p><b>Chapter 11 Industrial enzymes: an introduction 311</b></p> <p>11.1 Sales value and manufacturers 313</p> <p>11.2 Sources and engineering 314</p> <p>11.3 Environmental benefits 315</p> <p>11.4 Enzyme detection and quantification 315</p> <p>11.5 Immobilized enzymes 316</p> <p>11.6 Extremophiles 319</p> <p>11.7 Enzymes in organic solvents 324</p> <p>11.8 Industrial enzymes: the future 325</p> <p>Further reading 325</p> <p><b>Chapter 12 Industrial enzymes: proteases and carbohydrases 327</b></p> <p>12.1 Proteolytic enzymes 327</p> <p>12.2 Carbohydrases 340</p> <p>Further reading 367</p> <p><b>Chapter 13 Additional industrial enzymes 371</b></p> <p>13.1 Lipases 371</p> <p>13.2 Penicillin acylase 375</p> <p>13.3 Amino acylase and amino acid production 378</p> <p>13.4 Cyclodextrins and cyclodextrin glycosyltransferase 380</p> <p>13.5 Enzymes and animal nutrition 382</p> <p>13.6 Enzymes in molecular biology 387</p> <p>Further reading 390</p> <p><b>Chapter 14 Non-catalytic industrial proteins 393</b></p> <p>14.1 Functional properties of proteins 393</p> <p>14.2 Milk and milk proteins 397</p> <p>14.3 Animal-derived proteins 408</p> <p>14.4 Plant-derived proteins 411</p> <p>14.5 Sweet and taste-modifying proteins 412</p> <p>Further reading 414</p> <p>Index 417</p>

<b>Gary Walsh</b>, University of Limerick, Ireland.

<i>Proteins Biochemistry and Biotechnology</i> 2e is a definitive source of information for all those interested in protein science, and particularly the commercial production and isolation of specific proteins, and their subsequent utilization for applied purposes in industry and medicine.<br /> <br /> Fully updated throughout with  new or fundamentally revised sections  on proteomics as, bioinformatics, protein glycosylation and  engineering,  well as sections detailing advances in upstream processing and  newer protein  applications such as enzyme-based biofuel production  this new edition has an increased focus on biochemistry to ensure the balance between biochemisty and biotechnology, enhanced with numerous case studies.<br /> <br /> This second edition is an invaluable text for undergraduates of biochemistry and biotechnology but will also be relevant to students of microbiology, molecular biology, bioinformatics and any branch of the biomedical sciences who require a broad overview of the various medical, diagnostic and industrial uses of proteins. <br /> <br /> • Provides a comprehensive overview of all aspects of protein biochemisty and protein biotechnology<br /> • Includes numerous case studies <br /> • Increased focus on protein biochemistry to ensure balance between biochemisty and biotechnology<br /> • Includes new section focusing on proteomics as well as sections detailing protein function and enzyme-based biofuel production<br /> <br /> "With the potential of a standard reference source on the topic, any molecular biotechnologist will profit greatly from having this excellent book. " (<i>Engineering in Life Sciences</i>, 2004; Vol 5; No. 5)<br /> <br /> “Few texts would be considered competitors, and none compare favorably." (<i>Biochemistry and Molecular Education</i>, July/August 2002) <br /> <br /> "...The book is well written, making it informative and easy to read..." (<i>The Biochemist</i>, June 2002)

GB